Using nearly 6 years of nighttime differential absorption lidar ozone and Rayleigh/Raman lidar temperature measurements obtained by the Jet Propulsion Laboratory at Mauna Loa Observatory, Hawaii, the stratospheric quasi-biennial oscillation (QBO) signatures (15–55 km) in ozone and temperature are revealed. Several clear QBO signatures have been observed in both temperature and ozone. The QBO signature in temperature maximizes at 35 km (5 K amplitude) and is seasonally synchronized (winter) and out of phase with the expected signature at the equator. Also, for the periods 1994–1996 and 1998–2000 the QBO is observable above 45 km, and near 24 km, with opposite phase to that observed at 35 km. The clearest QBO signature in stratospheric ozone maximizes near 31 km and is also seasonally synchronized in late winter-early spring and is out of phase with the expected equatorial ozone QBO anomaly. Other signatures are observed in winter-spring near 47 km, in summer near 26 km, and in winter-spring near 22 km, in phase, in phase, and out of phase, respectively, with the expected equatorial ozone QBO. These QBO anomalies identified in ozone and temperature are highly consistent with the previously observed and modeled QBO anomalies assuming that Hawaii is located in the subtropical branch of the asymmetrical QBO-induced meridional circulation. The observed temperature and ozone QBO were both strongly disturbed by the El Nino-Southern Oscillation below 25–27 km in 1997–1998.