In marine sedimentary environments, microbial methanotrophy represents an important sink for methane before it leaves the seafloor and enters the water column. Using benthic observatories in conjunction with numerical modeling of pore water gradients, we investigated seabed methane emission rates at cold seep sites with underlying gas hydrates at Hydrate Ridge, Cascadia margin. Measurements were conducted at three characteristic sites which have variable fluid flow and sulfide flux and sustain distinct chemosynthetic communities. In sediments covered with microbial mats of Beggiatoa, seabed methane efflux ranges from 1.9 to 11.5 mmol m⁻² d⁻¹. At these sites of relatively high advective flow, total oxygen uptake was very fast, yielding rates of up to 53.4 mmol m⁻² d⁻¹. In sediments populated by colonies with clams of the genus Calyptogena and characterized by low advective flow, seabed methane emission was 0.6 mmol m⁻² d⁻¹, whereas average total oxygen uptake amounted to only 3.7 mmol m⁻² d⁻¹. The efficiency of methane consumption at microbial mat and clam field sites was 66 and 83%, respectively. Our measurements indicate a high potential capacity of aerobic methane oxidation in the benthic boundary layer. This layer potentially restrains seabed methane emission when anaerobic methane oxidation in the sediment becomes saturated or when methane is bypassing the sediment matrix along fractures and channels.