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AGU: Paleoceanography

 

Keywords

  • benthic foraminifera
  • viability assay
  • stable isotopes
  • epifluorescence microscopy

Index Terms

  • Paleoceanography: Micropaleontology (0459, 3030)
  • Paleoceanography: Instruments and techniques
  • Biogeosciences: Biosignatures and proxies

Abstract

PALEOCEANOGRAPHY, VOL. 21, PA4210, 8 PP., 2006
doi:10.1029/2006PA001290

Comparison of two methods to identify live benthic foraminifera: A test between Rose Bengal and CellTracker Green with implications for stable isotope paleoreconstructions

Joan M. Bernhard

Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts, USA

Dorinda R. Ostermann

Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts, USA

David S. Williams

Department of Geography, Geology, and Anthropology, Indiana State University, Terre Haute, Indiana, USA

Jessica K. Blanks

Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, South Carolina, USA

The conventional method to distinguish live from dead benthic foraminifers uses Rose Bengal, a stain that reacts with both live and dead cytoplasm. CellTracker Green CMFDA is a fluorogenic probe causing live cells to fluoresce after proper incubation. To determine the more accurate viability method, we conducted a direct comparison of Rose Bengal staining with CellTracker Green labeling. Eight multicore tops were analyzed from Florida Margin (SE United States; 248–751 m water depths), near Great Bahama Bank (259–766 m), and off the Carolinas (SE United States; 220 and 920 m). On average, less than half the Rose Bengal–stained foraminifera were actually living when collected. Thus, while Rose Bengal can significantly overestimate abundance, combined analyses of CellTracker Green and Rose Bengal can provide insights on population dynamics and effects of episodic events. Initial stable isotope analyses indicate that the CellTracker Green method does not significantly affect these important paleoceanographic proxies.

Received 1 March 2006; accepted 21 July 2006; published 1 December 2006.

Citation: Bernhard, J. M., D. R. Ostermann, D. S. Williams, and J. K. Blanks (2006), Comparison of two methods to identify live benthic foraminifera: A test between Rose Bengal and CellTracker Green with implications for stable isotope paleoreconstructions, Paleoceanography, 21, PA4210, doi:10.1029/2006PA001290.

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