High-resolution Doppler ionograms taken at 5 min intervals were obtained from a KEL IPS 71 ionosonde operating over a full ionosonde sweep range. The ionograms were converted into true height profiles using the program POLAN. POLAN also produced an equivalent parabolic layer model of best fit to the true height profile. A parabolic layer model of the ionosphere is defined by three parameters, namely, peak height, maximum electron density/critical frequency, and parabolic thickness. Equations for calculating Doppler shift from a time-varying parabolic layer model have long been known but have seen remarkably little use in the absence of suitable input data and a means of verifying the results. This paper shows that these equations can provide an accurate means of calculating ionospheric Doppler shift based on standard ionospheric ionograms taken at a 5 min rate over a 24 h period when compared with actual Doppler measurements. Apart from its scientific interest, the demonstrated technique will prove particularly valuable in deriving Doppler shift and associated signal fading for the real-time control of over-the-horizon radar and HF communication links.